Figure 4.

miR-140-5p targeted CTSB transcription. A: the downstream target genes of miR-140-5p were predicted through starbase (http://starbase.sysu.edu.cn/), jefferson (https://cm.jefferson.edu/rna22/precomputed/), and RNAInter (http://www.rna-society.org/raid/search.html) websites. B: the binding site between miR-140-5p and CTSB was predicted through starbase website. C: the binding relationship between miR-140-5p and CTSB was verified using dual-luciferase assay. D: CTSB expression was detected using qRT-PCR. the cell experiment was repeated 3 times independently. data in panel B (left) are enumeration data; data in panels B (right) and C are measurement data and expressed as mean ± standard deviation. data in panel C were analyzed using two-way ANOVA, and data in panel D were analyzed using one-way ANOVA, followed by Tukey’s multiple comparisons test, ***p < 0. 001