Figure 5.

Upregulation of CTSB attenuated the inhibitory effect of miR-140-5p overexpression on chondrocyte pyroptosis. the OA cells with high expression of miR-140-5p were transfected with pcDNA3.1-CTSB, with pcDNA3.1-NC as the control. A: CTSB expression was detected using qRT-PCR and western blot. B: Pyroptosis was measured using calcein-AM/PI staining. C: GSDMD-N protein level was detected using western blot. D: cleaved caspase-1, IL-1β, IL-18, and ROS levels were detected using the kits. the cell experiment was repeated 3 times independently. the measurement data are expressed as mean ± standard deviation and analyzed using one-way ANOVA, followed by Tukey’s multiple comparisons test, ***p < 0. 001