Figure 7.

Upregulation of miR-140-5p reduced chondrocyte pyroptosis by inhibiting the binding of CTSB/NLRP3. the OA cells with high expression of miR-140-5p were treated with Nigericin sodium salt (NSS), an agonist of NLRP3, with DMSO as the control. A: CTSB and NLRP3 protein levels were detected using western blot. B: Pyroptosis was measured using calcein-AM/PI staining. C: cleaved caspase-1, IL-1β, IL-18, and ROS levels were detected using the kits. the cell experiment was repeated 3 times independently. The measurement data are expressed as mean ± standard deviation. data in panel A were analyzed using two-way ANOVA, and data in panels B/C were analyzed using one-way ANOVA, followed by Tukey’s multiple comparisons test, ***p < 0. 001