Figure 1.

Curdione combined with DTX enhanced the inhibitory effects on cell proliferation in MDA-MB-468.(a) cell viability of MDA-MB-468 treated with different concentrations of Curdione (0, 2.5, 5, 10, 20, 40, 80 µM) for 48 h was performed by CCK-8. IC₅₀ = 151.712 µM. * p < 0.05, ** p < 0.01. (b) The combination index (CI) of the combinative treatment of curdione and docetaxel on MDA-MB-468 was determined by Chou-Talalay method using CompuSyn software. the IC₅₀ value of docetaxel for MDA-MB-468 was 0.911 µg/ml. the best synergetic effect of the combinative treatment of Curdione and docetaxel on MDA-MB-468 was gained from the treatment of 40 µM curdione with 1 µg/ml docetaxel for 48 h, of which CI is 0.43962. (c) the cell viability treated with Curdione (40 µM) and/or DTX (1 µg/ml) was measured in MDA-MB-468 and MCF10A cells by CCK-8. (d) BrdU staining was performed in MDA-MB-468 cells treated with Curdione (40 µM) and/or DTX (1 µg/ml). THE positive BrdU cells were calculated. *p < 0.05, **p < 0.01 versus the control group