Figure 4.

Co-treatment of Curdione and DTX affects MAPK signaling pathway in MDA-MB-468 cells. (a) MAPK signaling pathway related proteins (NF-κB, p38, p-p38, Erk1/2, p-Erk1/2) were detected in MDA-MB-468 cells by western blot. cells were treated with Curdione, DTX, Curdione and DTX, Curdione and DTX plus NAC. (b) The relative protein expression of these related proteins was determined by Image J software. (c) the expression levels of p38, p-p38, Bcl-2, cytochrome c were detected with the treatment of Curdione, DTX, Curdione and DTX, Curdione and DTX plus SB203580 (p38 inhibitor). GAPDH was used as an internal control. (d) the relative protein expression of these related proteins was determined by Image J. *p < 0.05, **p < 0.01