Figure 5.

Co-treatment of Curdione and DTX affects PI3K/Akt/p21 signaling in MDA-MB-468 cells. PI3K/Akt signaling pathway related proteins (Akt, p-Akt, p21, p27) were detected in MDA-MB-468 cells by western blot. Cells were treated with Curdione, DTX, Curdione and DTX, Curdione and DTX plus NAC. (b) The relative protein expression of these related proteins was determined by Image J. (c) Expression levels of Akt, p-Akt, p21, p27 were detected with the treatment of Curdione, DTX, Curdione and DTX, Curdione and DTX plus SC-79 (Akt activator). GAPDH was used as an internal control. (d) The relative protein expression of these related proteins was determined by Image J.*p < 0.05, **p < 0.01