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. 2021 Dec 7;12(2):10734–10744. doi: 10.1080/21655979.2021.2003129

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Overexpression of BBC3 reversed the effects of HOTAIR knockdown expression. The mRNA (a) and protein (b-c) expression of BBC3 was detected by pRT-PCR and western blot, respectively. (d) The cell apoptosis was detected by flow cytometer, and the apoptotic rates were calculated (e). The mRNA (f) expression of caspase-3 and the protein (g-h) expression of cleaved caspase-3 were detected by pRT-PCR and western blot, respectively. All experiments were performed in triplicate and data are presented as the mean ± standard deviation. *P < 0.05 and **P < 0.01