Figure 2.

Astragalus polysaccharide (APS) promoted cell survival, insulin secretion, PDX1 mRNA expression and inhibited cell apoptosis in HG+PA-treated MIN6 cells. (a) Proliferation was measured by CCK8 after transfection at 24, 48, 72 h, then the survival rate was calculated according to the OD value of NC inhibitor group. (b) Insulin secretion was measured by ELISA after transfection at 48 h. (c) PDX1 mRNA expression was measured by RT-qPCR after transfection at 48 h. (d-e) Apoptosis was measured by flow cytometry after transfection at 48 h. ^#P < 0.05, model group vs blank group; *P < 0.05, vs model group; ^&P < 0.05, vs 50 μg/ml astragalus polysaccharide treatment group