Figure 6.

A20 inhibited TNF-α, IL-6, and IL-1β expressions in microglial cells treated with 100 ng/mL LPS (n = 3). Both qPCR assay (a) and western blot assay (b) were adopted for the detection of A20 mRNA and protein expression, respectively. (c-e) mRNA levels of TNF-α, IL-6, and IL-β were subjected to qPCR. (f) Protein levels of TNF-α, IL-6, and IL-β were subjected to western blot. The relative density of TNF-α, IL-6, IL-1β, and A20 proteins was measured by Image J. (g) Apoptosis of microglia was detected by flow cytometry. OE-NC: overexpression negative control; si-NC: siRNA negative control. **, p < 0.01 compared with the negative control