Figure 3.

MiR-23a-3p directly targets CCL22. (a). The detection of CCL22 in GC and adjacent normal tissues via RT-qPCR; (b). The detection of CCL22 in GC cell lines (MGC-803, BGC-823, SGC-7901, MKN-45, MKN-7) and gastric mucosa epithelial cells GES-1 via RT-qPCR and Western blot; (c). The detection of CCL22 in MGC-803 cells transfected with miR-23a-3p mimic/inhibitor via RT-qPCR and Western blot; (d). The potential binding sites of miR-23a-3p and CCL22 predicted via TargetScan database (http://www.targetscan.org); (e). The targeting of miR-23a-3p with CCL22 verified via the luciferase report assay; The values were shown as mean ± SD (n = 3); Vs the mimic NC, *P < 0.05; Vs the inhibitor NC, ^ P < 0.05