Table 1.
X-ray diffraction statistics
| Fab:CGRP | Fab | |
|---|---|---|
| Wavelength (Å) | 0.95372 | 0.97917 |
| Space group | C2 | P21221 |
| Unit cell parameters (a, b, c in Å; α, β, γ in °) |
a = 68.235, b = 207.104, c = 68.204; α = γ = 90.00, β = 91.08 |
a = 62.781, b = 83.485, c = 90.190; α = β = γ = 90.00 |
| Resolution (Å) | 48.53–3.10 (3.31–3.10) | 50.00–1.54 (1.60–1.54)* |
| Measured reflections | 66,115 (11,076) | 423,952 |
| Unique reflections | 17,052 (2,996) | 70,440 (6,946) |
| Rsym | 17.4 (64.6) | 0.054 (0.823) |
| Completeness (%) | 99.2 (96.7) | 99.8 (99.9) |
| Average I/σ | 6.0 (1.7) | 21.7 (1.4) |
| Redundancy | 3.9 (3.7) | 6.0 (5.7) |
| CC1/2 | 0.984 (0.665) | 0.999 (0.639) |
| Fab molecules per asymmetric unit | 2 | 1 |
*Highest resolution shell of 3.31–3.10 Å (Fab:CGRP) and 1.60–1.54 Å (Fab).
Abbreviations: CC1/2, correlation coefficient determined by two random half-sets of data; CGRP, calcitonin gene-related peptide; Fab, antigen-binding fragment; Rsym, agreement between symmetry equivalent reflections.