Figure 5.

CAFs intensified the malignant behaviors of GC cells a. Both NFs and CAFs were isolated, and cellular immunofluorescence was carried out to identify the NFs and CAFs (marked by S110A4). b. WB verified fibroblast markers α-SMA, Vimentin, FSP and S110A4 in NFs and CAFs. NFs and CAFs were co-cultured with GC cells (AGS and SGC-7901). c. CCK-8 validated the proliferation of AGS and SGC-7901 cells. d. Transwell assay checked the migrative and invasive abilities of AGS and SGC-7901 cells. e. WB monitored the expression of EMT-related markers E-cadherin, ZO-1, Vimentin, N-cadherin, Snail, Slug and MMP3 in GC cells. f-h. RT-PCR gauged the NORAD/miR-496/IL-33 expression in AGS and SGC-7901 cells. i. ELISA detected IL-33 profiles in the culture medium. N = 3. ns, *,**, *** represents P > 0.05, P < 0.05, P < 0.01, P < 0.001