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. 2022 Jan 12;602(7895):101–105. doi: 10.1038/s41586-021-04269-6

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© The Author(s) 2022, corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 7 — a, Distribution of epigenomic features in genes with different numbers of exons. b, Epigenome-predicted Mutation Probability Score (MPS), rates of natural polymorphism, and Tajima’s D in genes with different numbers of exons. c, Left: comparison of Mutation Probability Score (MPS) between genes with UTRs and those lacking 5’ or 3’ UTRs. Horizontal lines mark the mean difference between genes with and without UTRs. Vertical lines mark the mean +- confidence intervals of two-sided t-tests. Center: rates of natural polymorphism in natural A. thaliana accessions. Right: Tajima’s D in natural accessions. (n = 35,526 gene models). d, Left: Pearson’s correlation coefficients for relationship between predicted mutation probabilities and the absolute length of 5’ and 3’ UTRs. Horizontal lines mark the means, and vertical lines mark the mean +- confidence intervals. Center: same for rates of natural polymorphism in natural accessions. Right: same for Tajima’s D in natural accessions. (n = 35,526 gene models). e, Left: Relationships between intron number and total intron length with predicted mutation probabilities. Points indicate mean values. Center: same for rates of natural polymorphism in natural accessions. Right: same for Tajima’s D in natural accessions. f, Results for 544 Populus trichocarpa accessions⁷⁵. Horizontal lines mark the means, and vertical lines mark the mean ± confidence intervals of two-sided t-tests (n = 73,013 gene models).