Figure 3.

Mic10^ATPsynthase restores physiological functions in mic10Δ cells

(A) EM analysis of yeast cells following diaminobenzidine staining.

(B) Quantitation of crista length in the indicated strains. Interquartile boxplot: first quartile; median; and third quartile are shown. N = 200.

(C) Live-cell fluorescence imaging of the mitochondrial network using MitoTracker dye in WT, mic10Δ, or atp21Δ cells with or without Atp21-Mic10 expression. DAPI, stain for mitochondrial nucleoids.

(D) Growth of WT cells as well as mic10Δ or atp21Δ cells with or without expression of Atp21-Mic10 in selective media with 3% glycerol + 0.1% glucose. Data are represented as mean $\pm$ SD; n = 4. OD, optical density.

(E) The mitochondrial membrane potential was assessed in isolated mitochondria by quenching of a fluorophore whose uptake depends on the membrane potential. Data are represented as mean $\pm$ SEM; n = 8 (upper panel); n = 14 (lower panel).

See also Figure S2.