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. 2022 Jan 11;25(2):103762. doi: 10.1016/j.isci.2022.103762

Figure 5.

Figure 5

Increased contact stimuli with the supply of microbeads restored locomotion activity and loss of physique under artificial microgravity

(A) D1 adults (vtIs1) grown in culture bags with or without microspheres under 3D clinorotation (Video S4).

(B–F) Expression levels of comt-4 by real-time RT-PCR with eft-2 internal standard, (C) endogenous DA levels, (D) maximal bending angle (n = 15 per condition), (E) moving frequency as thrashing rate (n = 30 per condition), and (F) body length (n = 30 per condition) was measured in day 1 adults (vtIs1) parallelly cultured for 4 days under normal gravity (1G) and simulated μG (3D) in the absence or presence of microspheres (‘+ beads’). Data are shown as box and whiskers to indicate median and standard deviations. Statistical analysis was performed in each condition using one-way ANOVA followed by Tukey post hoc test. Different letters indicate statistically significant differences at p< 0.05.