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. 2022 Jan 20;11:812549. doi: 10.3389/fonc.2021.812549

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Copyright © 2022 Yang, Xie, Zhang, Gu, Yuan, Wang, Guo, Ye, Peng, Yao and Yang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Representative fluorescence images of three types of CTCs isolated from the peripheral blood of omHSPC patients based on RNA-ISH staining for leukocytes (CD45, white), epithelial cells (EpCAM and CK8/18/19, red), and mesenchymal cells (vimentin and twist, green). 4´,6-Diamidino-2-phenylindole was used to stain cell nuclei (blue). The scale bar indicates 5 μm. (B) Levels of CTC subtypes. (C) The distribution of three subtypes of CTCs in each patient.