REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
Acetone ACS reagent, ≥99.5%	Sigma-Aldrich Inc, St. Louis, MO, USA	Cat. #: 179124
AnaSed xylazine injection	Akorn Inc, Lake Forest, IL, USA	NDC: 59399-110-20
Bovine Serum Albumin (BSA)	Fisher BioReagents, Waltham, MA, USA	Cat. #: BP9700100
Cyanine5.5 carboxylic acid	Lumiprobe Corporation, Hunt Valley, MD, USA	Cat. #: 47090
Dimethyl Sulfoxide (DMSO)	Santa Cruz Biotechnology, Dallas, TX, USA	Cat. #: sc-358801
Eosin Y	Fisher Scientific, Hampton, NH, USA	Item #: 17372-87-1
Gill III Hematoxylin	Mercedes Scientific, Lakewood Ranch, FL, USA	Item #: MER 347961GL
Phloxine B	Electron Microscopy Sciences, Hatfield, PA, USA	Item #: 19350
10X Phosphate-Buffered Serum	Invitrogen, Waltham, MA, USA	Cat. #: AM9624
Poly(D,L-lactide-co-glycolide)	Sigma-Aldrich Inc, St. Louis, MO	SKU: P2191
Poly(lactide-co-glycolide)-Fluorescein	Sigma-Aldrich Inc, St. Louis, MO	SKU: 908649-50MG
Refresh Liquigel Lubricant Eye Gel	Allergan, Irvine, CA, USA	NDC: 0023-9205
Refresh Tears Lubricant Eye Drops	Allergan, Irvine, CA, USA	NDC: 0023-0798-01
Tissue-Tek O.C.T. Compound	VWR International, LLC, Radnor, PA, USA	Cat. #: 4583
Tropicamide ophthalmic solution (1%)	Akorn Inc, Lake Forest, IL, USA	NDC: 17478-102-12
VetaKet CIII (ketamine hydrochloride injection, USP)	Akorn Inc, Lake Forest, IL, USA	NDC: 59399-114-10
Experimental models: Organisms/strains
Strain: WT BALB/c mouse Gender: male/female Age: 4–8 weeks	Envigo, USA	N/A
Strain: WT C57BL/6J mouse Gender: male/female Age: 4–8 weeks	The Jackson Laboratory, USA	Stock #: 000664
Software and algorithms
Aura imaging software	Spectral Instruments Imaging, Tucson, AZ, USA	https://spectralinvivo.com/
GraphPad Prism 8	GraphPad Software, Inc.	https://www.graphpad.com/
ImageJ	National Institutes of Health (NIH)	https://imagej.nih.gov/ij/index.html
Leica Application Suite X	Leica Microsystems, San Francisco, CA, USA	https://www.leica-microsystems.com/products/microscope-software/p/leica-las-x-ls/
Other
Black Arkansas stone	Dan's Whetstone Company Inc., AR, USA	N/A
Borosilicate glass capillaries	World Precision Instruments, FL, USA	Cat. #: 1B100-4
Capillary holder	Eppendorf, Hamburg, Germany	Cat #: 920007392
Customized +10D contact lens (3.0 mm diameter, 1.6 mm BC, PMMA clear)	Advanced Vision Technologies, CO, USA	N/A
Diagnosys ERG Celeris	Diagnosys LLL, Littleton, MA, USA	Celeris Model #: D430
Dual-Stage Glass Micropipette Puller	Narishige international USA, NY, USA	Model PC-10
Femtojet Express Electronic microinjector	Eppendorf, Hamburg, Germany	Cat #: 920010521
Foot control pedal	Eppendorf, Hamburg, Germany	Cat #: 920005098
Heating pad	K&H Manufacturing, CO, USA	Model HM10
Heidelberg OCT Spectralis	Heidelberg Engineering, Germany	N/A
Leica DM4000 B LED automated upright microscope system	Leica Microsystems, San Francisco, CA	N/A
Leica M165 FC fluorescent stereo microscope	Leica Microsystems, San Francisco, CA	N/A
Leica MZ6 modular stereomicroscope	Leica Microsystems, San Francisco, CA	N/A
Micropipette Grinder	Narishige international USA, NY, USA	Model EG-401
Near-infrared machine	Spectral Instruments Imaging, Tucson, AZ, USA	Lago X system
BD Precisionglide needles, 20 gauge	Sigma-Aldrich Inc, St. Louis, MO, USA	Cat #: Z192511
BD Hypodermic Syringe, polypropylene, 10 mL	Sigma-Aldrich Inc, St. Louis, MO, USA	Cat #: Z192023
Whatman PURADISC 25, Polyether Sulfone Membrane Filter, 0.2 μm	GE Healthcare, Chicago, IL, USA	Cat #: WHA67802502
Positioning aids	Eppendorf, Hamburg, Germany	Cat #: 920005829
Spark plate reader	Tecan, Morrisville, NC, USA	N/A
Syringe cleaning wire	Hamilton Company, Reno, NV, USA	Part/REF #: 18300
VWR micro cover glass	VWR International, LLC, Radnor, PA, USA	Cat. # :48393-081
VWR Superfrost Plus Micro Slide	VWR International, LLC, Radnor, PA, USA	Cat. #: 48311-703