Phenotype of WT mice fed with HFD. C57BL/6 mice were fed with NCD or HFD for 16 weeks (n = 3–5). A-B: Body weight (A) and liver weight (B) were measured. C-H: Overnight-fasted serum ALT levels (C), AST levels (D), TC levels (E), TG levels (F), glucose levels (G), and insulin levels (H) were measured. I-J: Representative images of H&E-stained (I) and Sirius red stained (J) livers. Scale bar, 100 μm. K: Representative images of Oil red O-stained livers. Quantification of Oil red O-stained area was shown in the right. Scale bar, 100 μm ∗P < 0.05 vs. NCD group.