Figure S2.

Phenotype of LKO mice fed with NCD. A: Schematic diagram showing the targeting strategy for generating LKO mice. B-C: The expression of hepatic CD36 was analyzed by Q-PCR (B) and immunoblotting (C). D: The expression of CD36 in heart, kidney, and adipose tissues was analyzed by immunoblotting. E: Quantitation of protein levels in (B) and (C). n=3, ∗P < 0.05 vs. fl/fl mice. F-P: The fl/fl and LKO mice were fed with NCD for 16 weeks (n=6-12). Body weight (F), daily intake (G), and liver weight (H) were measured. Overnight-fasted serum glucose levels (I), TG levels (J), TC levels (K), as well as ALT (L) and AST levels (M) were measured. Representative images of H&E-stained livers (N). Scale bar, 100 μm. GTTs (O) and ITTs (P) in NCD-fed mice, respectively. Quantification of AUC was shown in the right.