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. 2021 Dec 30;57:101428. doi: 10.1016/j.molmet.2021.101428

Figure 1.

Figure 1

Hepatic CD36 is regulated by nutritional signals. A–B: C57BL/6J male mice were fed with either the normal chow diet (NCD) or high-fat diet (HFD) for 16 weeks. The hepatic expression of CD36 was analyzed by immunoblotting (A) or real-time quantitative PCR (Q-PCR) (B). n = 3, ∗P < 0.05 vs. NCD group. C: The hepatic CD36 mRNA levels in the nonalcoholic fatty liver disease group (NAFLD, n = 40) and normal control group (control, n = 21) from the GEO database (GSE151158). ∗P < 0.05 vs. control group. D–E: C57BL/6J male mice were overnight-fasted and then fed again 12 h. Livers were collected and the protein level of CD36 (D) and SREBP1 signaling (E) were analyzed by immunoblotting. n = 3, ∗P < 0.05 vs. fasted group. F: C57BL/6J male mice were fasted overnight and injected with PBS or insulin (1U/kg body weight) for 4 h. Livers were collected and the protein level of CD36 was analyzed by immunoblotting. n = 3, ∗P < 0.05 vs. PBS group. G: HepG2 cells were treated with insulin (100 nM) for indicated time points. The protein levels of CD36 were analyzed immunoblotting. n = 3.