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. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: J Cell Physiol. 2021 Aug 8;237(1):720–729. doi: 10.1002/jcp.30541

Figure 1. Klotho deficiency caused severe arterial calcification and increased the protein levels of BMP2, BMP4, and RUNX2 in aortas.

Figure 1.

(A), H&E and Alizarin-Red staining of thoracic aorta tissue sections (original magnifications: ×200 and x100) from 8-week-old wt and kl/kl deficient mice. (B), The area fraction of Alizarin-Red positive-staining in aorta section (n=5). ***p<0.001, compared with wt mice. (C), Western blot analysis of BMP2 in aortas. (D), Western blot analysis of BMP4 and RUNX2 in aortas. n=5; *p<0.05, **p<0.01 vs 4-week-old wt; +p<0.05, +++p<0.001 vs 6-week-old wt; #p<0.05 vs 8-week-old wt. Data=means±SEM.