Figure 6. Klotho-deficient serum promoted BMP2-VitD3-induced calcification in cultured mouse aortic smooth muscle cells (MOVAS).

Confluent cells were incubated with 2.5% normal FBS or 2.5% KL-deficient FBS in the presence or absence of 1 nM of vitamin D3 or 200 ng/mL of BMP2 for two weeks. Culture medium was changed every 3 days. Representative photographs of Alizarin Red staining at the magnification of 100 (A). Quantification of calcium contents in extracellular matrix (B). n=4 independent experiments. *p<0.05, ***p<0.001 vs the control; ^p<0.05, ^^^p<0.001 vs VitD3-BMP2. Data=means±SEM.