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. 2022 Jan 20;12:758829. doi: 10.3389/fimmu.2021.758829

Figure 4.

Figure 4

SPRR3 knockdown inhibits the expression of IL-33 and ILC2 accumulation in mice. (A) The mRNA expression of IL-33 measured using quantitative PCR. (B) The protein levels of IL-33 in bronchoalveolar lavage fluid (BALF) determined by ELISA. (C) Western blotting analysis of AKT and NF-κB signaling pathway activation. (D) Quantification of phosphorylation AKT normalized to total AKT, shown as relative expression. (E) Quantification of phosphorylation NF-κB P65 normalized to total NF-κB P65, shown as relative expression. (F) Flow cytometric dot plots of ST2+Thy1.2+ILC2s gated on CD45+LIN− cells. (G) Percentage of ILC2 in the lung. (H) ILC2 numbers in the lung. Values represent mean ± SEM, n = 4–6 for each group. * p < 0.05, ** p < 0.01.