FIGURE 2.

Influence of BZR1 on photosynthesis in tomato plants. (A) Induction of dephosphorylation of BZR1 by endogenous BRs. The phosphorylated and dephosphorylated forms of BZR1 are termed by pBZR1 and dBZR1, respectively. Total proteins were probed using an anti-BZR1 multiclonal antibody. The ratio reflects the relative abundance of dBZR1 to total protein, expressed as a number. Actin was used as the loading control. The signal intensity of dBZR1 protein was analyzed with Image Lab. The dBZR1 level of WT plants was set as one. (B) Net photosynthetic rate (Pn). (C) The actual quantum yield of PSII (Φ_PSII). (D) Photochemical quenching coefficient (qP). (E) Maximum ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) carboxylation rate (V_c,max). (F) Maximum ribulose-1,5-bisphosphate (RuBP) regeneration rate (J_max). Five-week-old bzr1 mutant and wild type (WT; “Condine Red”) plants were used for this analysis. EBR (200 nM) was applied at 8:00 in the morning. For (B–D), values were measured 24 h after EBR treatment. For (E,F), values were measured 48 h after EBR treatment. For (B–F), the data represent the means ± SD of at least three biological replicates. Different letters represent significant differences (p < 0.05) according to Tukey’s test. Three independent experiments were performed with similar results.