Skip to main content
. 2022 Jan 20;12:811948. doi: 10.3389/fpls.2021.811948

FIGURE 4.

FIGURE 4

BZR1 binds to the promoters of FBA1, RCA1, FBP5, and PGK1 in vitro and in vivo. (A) E-box elements in the promoters of FBA1, RCA1, FBP5, and PGK1 genes. The numbering is counted from the predicted transcriptional start sites. (B) Yeast one-hybrid (Y1H) experiment showing the binding of BZR1-AD to the promoters of FBA1, RCA1, FBP5, and PGK1. Binding sites of every gene were shown in (A). (C) Dual-luciferase assay for the regulatory effect of BZR1 on the expression of FBA1, RCA1, FBP5, and PGK1. The ratio of LUC/REN of the combination of empty SK vectors mixed with the FBA1, RCA1, FBP5, and PGK1 promoters was set as one. (D) Direct binding of BZR1 to the promoters of FBA1, RCA1, FBP5, and PGK1 by ChIP–qPCR in 35S-BZR1-HA overexpressing (BZR1: OE) plants. Data present the means of three replicates ± SD. Different letters indicate significant differences (p < 0.05) according to Tukey’s test. Three independent experiments were performed with similar results.