Evaluation of 89Zr-DFO-AF650 imaging metrics for Trop-2 immunoPET among pancreatic cancer models. a The correlation between ROI and BioD (%ID/g); ORT, orthotopic model; S.C., subcutaneous model. b–c The Z-normalized heat map of 89Zr-DFO-AF650 accumulated values in various organs via specified ROI (%ID/g) at 4 and 24 h post injection; groups including A: AF650 (AsPC-1, S.C.), B: AF650 (BxPC-3, S.C.), I: IgG (BxPC-3, S.C.), M: AF650 (MIA PaCa-2, S.C.), O: AF650 (BxPC-3, ORT)