Fig. 6. SpvB promotes necroptosis of IECs in vivo.

A, B C57BL/6 mice were treated with streptomycin 24 h prior to oral infection with the WT or ΔspvB S. Typhimurium strain (1 × 10⁸ CFUs) and analyzed at 48 h. IHC staining evaluated the level of A p-MLKL and B RIPK3 in colon sections. Scale bars: 50 μm. C, D Western blot analysis of purified colonic epithelial lysates with specific antibodies to C p-MLKL and D RIPK3, n = 3 mice for control group, n = 4 mice for WT- and ΔspvB-infected group respectively. Data were analyzed with IBM SPSS Statistics 19 and presented as the mean ± SEM using Student’s t-test. *P < 0.05.