Table 1.
Kinetic parameters of recombinant CAZymes.
| Protein name | CAZy family | Substrate | pH | T (°C) | Km | kcat (s−1) | kcat/Km |
|---|---|---|---|---|---|---|---|
| CapGH97 | GH97 | pNP-α-d-Gala | 7.0 | 35 | 7.70 ± 0.61 (mM) | 16.58 ± 0.49 | 2.15 |
| CapGH10 (full-length) | GH10 | Rye arabinoxylan | 5.0 | 50 | 2.09 ± 0.31 (mg mL−1) | 162.74 ± 11.34 | 77.87 |
| Beechwood xylan | 5.5 | 55 | 1.61 ± 0.10 (mg mL−1) | 16.96 ± 0.46 | 10.53 | ||
| CapGH10T (truncated GH10 catalytic domain) | GH10 | Rye arabinoxylan | 5.5 | 55 | 1.76 ± 0.13 (mg mL−1) | 206.76 ± 6.85 | 117.48 |
| Beechwood xylan | 5.5 | 55 | 1.75 ± 0.18 (mg mL−1) | 79.48 ± 3.16 | 45.42 | ||
| CapGH43_12 | GH43_12 | pNP-α-l-AraF | 6.5 | 35 | 2.74 ± 0.29 (mM) | 151.19 ± 6.21 | 55.18 |
| CapGH173 | GH173 | pNP-β-d-Gal | 7.5 | 45 | 0.57 ± 0.05 (mM) | 17.6 ± 0.39 | 30.88 |
| BXY_26070 (CBK67650) | GH173 | pNP-β-d-Gal | 7.5 | 45 | 1.19 ± 0.35 (mM) | 29.85 ± 1.95 | 25.08 |
The pH, temperature, and substrate saturation curves are shown in Supplementary Figs. 7, 12–14. The kinetic parameters, kcat (turnover number) and Km (Michaelis constant) are expressed as mean ± SD from three independent experiments (n = 3).
aKinetic parameters determined using 5 mM of CaCl2. Source data are provided as a source data file.