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. 2021 Sep 1;126(3):482–491. doi: 10.1038/s41416-021-01538-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Representative 1D plots obtained by ddPCR analysis showing fluorescence produced by patient-specific probes and copy numbers in 10-fold serial dilutions of a ALL, b HR-NB and c EWS patient DNA. The FAM fluorescent signal (y-axis) of each droplet is plotted against the droplet cumulative count (x-axis). Each dot above the threshold line shows a positive droplet for the patient-specific DNA breakpoint target. d–f Linear regression standard curves obtained by log 2 transformation of ddPCR copy numbers in serial dilutions of ALL, HR-NB and EWS DNA.