TABLE 1.

Results of follow-up surveys of the patient and the donor

Sample source and date (yr-mo-day)	Detection of parasites			Reciprocal IFA titers
	Microscopya	PCRb	Inoculationc	IgM	IgG
Patient
1998-12-28d	NDe	−	ND	<100	<100
1999-05-24	+	+	+	12,800	1,600
1999-06-25	−	+	ND	12,800	800
1999-07-27	−	+	+	3,200	1,600
1999-08-31	+	+	+	12,800	102,400
1999-10-02	−	+	ND	12,800	51,200
1999-11-01	ND	−	ND	1,600	25,600
1999-11-29	ND	−	ND	800	25,600
1999-12-27	ND	−	ND	400	6,400
2000-01-29	ND	ND	ND	200	3,200
2000-03-08	ND	−	ND	200	3,200
2000-05-22	−	−	−	200	1,600
2000-07-19	−	−	−	100	800
Donor
1998-12-22	ND	+	ND	200	25,600
1999-06-26	ND	−	ND	100	25,600
1999-07-27	−	−	+	50	25,600
1999-08-31	−	+	+	25	12,800
1999-10-02	−	+	+	50	25,600
1999-11-11	−	−	+	200	51,200
1999-12-20	−	−	−	100	51,200
2000-01-31	−	−	+	50	25,600
2000-03-06	−	−	+	100	25,600
2000-04-24	ND	+	ND	ND	ND

^aGiemsa-stained thin-smeared blood films were microscopically observed; +, parasitemia detectable; −, not detectable. 

^bAmplification by nested PCR. Results were confirmed in different laboratories by independent tests with different primers sets; +, amplification positive; −, negative. 

^cInoculation into splenectomized hamster. Blood samples from the animals were observed for 2 months; +, parasitemia detected; −, not detected. 

^dBecause a blood sample from the patient was not available on this day, the results were estimated with extracts from the cross-match test papers made prior to blood transfusion. 

^eND, not done.