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. 2022 Feb 2;13(2):99. doi: 10.1038/s41419-022-04575-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — (A–B) Endogenous TKT coimmunoprecipitates with GRP78 in SW480 and HCT116. (C) The regulation of TKT to AKT phosphorylation was dependent on GRP78. (D–E) Regulation of TKT to aerobic glycolysis is dependent on GRP78. (F) Relative glucose consumption was determined in HCT116 cells. 12 h: Ctrl vs. TKT-Flag: *; TKT-Flag vs TKT-Flag +NC: n.s; TKT + NC vs TKT + shGRP78-1: **, TKT + NC vs TKT + shGRP78-2: **. 24 h: Ctrl vs. TKT-Flag: **; TKT-Flag vs TKT-Flag +NC: n.s; TKT + NC vs TKT + shGRP78-1: *, TKT + NC vs TKT + shGRP78-2: *. 36 h: Ctrl vs. TKT-Flag: *; TKT-Flag vs TKT-Flag +NC: n.s; TKT + NC vs TKT + shGRP78-1: *, TKT + NC vs TKT + shGRP78-2: *. 48 h: Ctrl vs. TKT-Flag: *; TKT-Flag vs TKT-Flag +NC: n.s; TKT + NC vs TKT + shGRP78-1: **, TKT + NC vs TKT + shGRP78-2: *. (G) Relative lactate productionwas determined in HCT116 cells. 12 h: Ctrl vs. TKT-Flag: *; TKT-Flag vs TKT-Flag +NC: n.s; TKT + NC vs TKT + shGRP78-1: **, TKT + NC vs TKT + shGRP78-2: *.24 h: Ctrl vs. TKT-Flag: **; TKT-Flag vs TKT-Flag +NC: n.s; TKT + NC vs TKT + shGRP78-1: *, TKT + NC vs TKT + shGRP78-2: *. 36 h: Ctrl vs. TKT-Flag: **; TKT-Flag vs TKT-Flag +NC: n.s; TKT + NC vs TKT + shGRP78-1: **, TKT + NC vs TKT + shGRP78-2: **. 48 h: Ctrl vs. TKT-Flag: *; TKT-Flag vs TKT-Flag +NC: n.s; TKT + NC vs TKT + shGRP78-1: **, TKT + NC vs TKT + shGRP78-2: *. (H) The content of F6P was detected in HCT116. (I) Western blot analysis about the expression of Glut1 and LDHA. (J–K) Migration and invasion detection with TKT overexpression and GRP78 knockdown. (L) Representative pictures and analysis of lung metastases in mice. (M) Corresponding H&E -stained lung sections. Arrows denote lung metastasis. *P < 0.05, **P < 0.01, ***P < 0.001.