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. 2022 Feb 2;12:1730. doi: 10.1038/s41598-022-05856-x

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(a) A 3D representation of the principal component analysis (PCA) of all individuals included in the study. Each color represents the cluster inferred from the structure analysis; C. albertensis (yellow), C. occidentalis (blue), C. sonorensis (teal), C. variipennis (red), and the unidentified San Diego population (green). Hybrids (h1–h7) are designated with a black circle and their inferred parental ancestry is depicted with pie charts. The geographic locations of the two C. occidentalis clusters are labeled next to each grouping (see Table 1 for abbreviation). (b) Unrooted maximum likelihood phylogenetic tree based on 199 individuals inferred from 3612 SNPs (the hybrids were removed here but are included in Fig. S3.). Clade colors also represent the clusters inferred from the structure analysis. Support values written on the branches: rapid bootstrap (%)/SH-aLRT support (%)/ultrafast bootstrap support (%). For clarity, the values within each cluster are not shown.