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. 2022 Feb 2;13(2):104. doi: 10.1038/s41419-022-04527-z

Fig. 5. Baicalein attenuates adenine-induced ferroptotic cell death in human primary PTEC via induction of SOD2.

Fig. 5

A Left panel: Fold changes (relative to 0 mM adenine without baicalein treatment) in SOD2 expression for PTEC cultured in the absence (0 mM) and presence (8 mM) of adenine, both without (-baicalein) and with (+baicalein) treatment. Bar graphs represent median values with interquartile range. Symbols represent individual donor PTEC; n = 6. *p < 0.05, Wilcoxon matched-pairs signed-rank test. Right panel: Representative SOD2 Western blot for PTEC cultured in the absence (0 mM) and presence (8 mM) of adenine, both without (-baicalein) and with (+baicalein) treatment (15 µg total protein per lane). B Left panel: Fold changes (relative to 0 mM adenine without baicalein treatment) in mitochondrial superoxide levels (measured as ΔMFI) for PTEC cultured in the absence (0 mM) and presence (8 mM) of adenine, both without (-baicalein) and with (+baicalein) treatment. Bar graphs represent median values with interquartile range. Symbols represent individual donor PTEC; n = 6. *p < 0.05, Wilcoxon matched-pairs signed-rank test. Right panel: Representative MitoSOX staining (black unfilled) compared with unstained control (grey filled) for PTEC cultured in the absence (0 mM) and presence (8 mM) of adenine, both without (-baicalein) and with (+baicalein) treatment. Mitochondrial superoxide levels (ΔMFI) are presented for each histogram, with fold change (FC) value relative to 0 mM adenine without baicalein treatment also shown. C Left panel: Fold changes (relative to 0 mM adenine without baicalein treatment) in γ-H2AX expression (measured as % cells with >5 γ-H2AX foci) for PTEC cultured in the absence (0 mM) and presence (8 mM) of adenine, both without (-baicalein) and with (+baicalein) treatment. Bar graphs represent median values with interquartile range. Symbols represent individual donor PTEC; n = 6. *p < 0.05, Wilcoxon matched-pairs signed-rank test. Right panel: Immunofluorescent microscopy of representative PTEC cultured in the absence (0 mM) and presence (8 mM) of adenine, both without (-baicalein) and with (+baicalein) treatment, and stained for γ-H2AX (green) and DAPI (blue). Scale bars represent 20μm.