Figure 11.

Estrous cycle-specific IPA enrichment analyses of differential phosphoprotein abundance following ketamine treatment in the female rat dHPC. Canonical pathway enrichment of phosphoproteins whose abundance was significantly altered by low-dose ketamine in proestrus (A) or diestrus (B) female rats. Based upon the direction of fold change of significant differentially abundant phosphoproteins within the dataset, the Z-Score serves as a predictor of pathway activation states by assessing the match between the observed and predicted (evidence-based) up/down regulation patterns⁴¹. Pathways are colored by predicted activation state: orange (activated) or blue (inhibited), where darker colors represent higher absolute Z-scores. Z-scores for pathways colored in gray could not be calculated due to insufficient evidence for a prediction of either activation or inhibition to be made. The ratio (solid orange line) represents the number of molecules within the comparison dataset meeting the cutoff criterion (p < 0.05; Fisher’s Exact Test), divided by the number of molecules comprising a given pathway within the reference set (Ingenuity Knowledge Base). Bar graphs were produced in Ingenuity Pathway Analysis (IPA) software (QIAGEN, Inc.).