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. 2022 Feb 2;12:1820. doi: 10.1038/s41598-022-05937-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Estrous cycle-dependent total and phosphoprotein expression in the dHPC. (A) Venn diagram showing number of total (“Total”) and phosphoproteins (“Phospho”) with significant differential abundance between female rats in either proestrus or diestrus. Red arrows indicate greater levels observed in proestrus vs. diestrus females. (B) Volcano plot depicting significant differences in protein and phosphoprotein abundance between proestrus and diestrus female rats. Log₂(Fold Change) values > 0 (red) indicate higher, and those < 0 (green) lower, abundance in proestrus females compared to diestrus females. Dots above the horizontal dashed line represent proteins with p < 0.05 (FDR threshold set at 1%). (C) Top 10 most significant (p < 0.05) gene ontology (GO) enrichment terms per category of proteins and phosphoproteins with significant estrous cycle-dependent abundance differences.