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. 2022 Feb 3;13:8. doi: 10.1007/s12672-022-00469-2

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Knockdown of CENPO inhibited CRC cells proliferation and arrested cell cycle in G2. The mRNA (A) and protein (B) expression levels of CENPO in HCT116 and RKO cells after lentiviral (shCtrl and shCENPO) interference were estimated respectively. C The effect of CENPO knockdown on HCT116 and RKO cell proliferation was detected using MTT assay. D Flow cytometry with PI staining was used to detect the effect of CENPO knockdown on the cell cycle distribution of HCT116 and RKO cells. The presented results were representative of experiments repeated at least three times. Data was represented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001