Fig. 4.

Knockdown of CENPO migration and invasion of CRC cells. The effect of CENPO knockdown on HCT116 and RKO cell migration (A) and invasion (B) was evaluated using wound-healing assay (A) and Transwell assay (B). C The expression of EMT-related protein of HCT116 and RKO cells with or without knockdown of CENPO by Western blot. D The expression of target proteins pathways was observed by Western blot in HCT116 and RKO cells. E The RKO cells with AKT activator on the basis of CENPO knockdown (shCENPO  +  AKT activator), which was used to detect the proliferation ability of cells. The presented results were representative of experiments repeated at least three times. Data was represented as mean  ±  SD. *P  < 0.05, **P  < 0.01, ***P  < 0.001