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. 2022 Feb 2;12:1763. doi: 10.1038/s41598-022-04868-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The absorbed dose, J/g, at the threshold for cell killing by uni- and bipolar pulses of different duration (solid and dashed lines, respectively). For bipolar pulses, the dose is plotted against the duration of one phase. The pulse shape and the number of pulses per train are labeled next to the respective curves (“10p”, “100p”, and “300p” stand for 10, 100, and 300 pulses per train respectively). Pulse repetition rate was 10 Hz. See text and Figs. 6 and 7 for more details.