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. 2022 Feb 2;12:1763. doi: 10.1038/s41598-022-04868-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Electric field simulations for the asymmetrical (A–C) and symmetrical (D–F) electrode assemblies. (A) and (D) show the spatial configuration of the electrode assemblies orthogonal to a plastic dish and touching its bottom. (B) and (E) show the calculated electric field distribution in a plane 5 µm above the bottom with 1 V applied between electrodes. The direction in which the electric field thresholds were measured is marked by vertical green lines (see text). (C) and (F) are the electric field values along the green lines. These values were linearly scaled to the voltage applied between the electrodes for calculation of the electric field thresholds.