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. 2022 Feb 2;12:1775. doi: 10.1038/s41598-022-05760-4

Figure 1.

Figure 1

SVF extraction and characterization. (A) The SVF was enzymatically isolated from human lipoaspirates using a collagenase-based enzymatic solution at 37 °C for 30 min at 120 rpm of reciprocating motion, followed by centrifugation at 800×g for 10 min. The open circle indicates the SVF pellet. (B) The freshly isolated SVF was characterized by the expression of CD45, CD34, CD31, CD105, CD146, CD157, and CD200 during the flow cytometry analysis. The experiment was performed independently 3 times (3 donors).