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. 2022 Feb 2;12:1775. doi: 10.1038/s41598-022-05760-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Time-dependent changes in AEPC cell morphologies and surface marker expression profiles. (A) The CD45⁻CD31⁺ and (B) CD45⁻CD31⁻ MACS-separated fractions were characterized for the expression of CD markers, including CD45, CD31, CD34, CD146, and CD105. The experiment was performed twice, independently from 2 donors. The graph indicates 2 donors, depicted by red and blue lines. The X-axis (passage number) indicates that the CD45⁻CD31⁺ fraction at P5 corresponded to dissociated AEPCs at P4D4, as shown in Fig. 3D.