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. 2022 Jan 20;12:778019. doi: 10.3389/fendo.2021.778019

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Copyright © 2022 Du, He, Xu, Qu, Cui, Zhang, Zhang, Li and Yu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Bone mass fluctuates with increased time. (A) Representative images of micro-CT are shown. (B) Trabecular bone microarchitecture of tibias showing bone volume/total volume (BV/TV), trabecular number (Tb.N), trabecular separation (Tb.Sp), and trabecular thickness (Tb.Th). Data are shown as the mean ± SD (n=5 per group). Significance (p-value) was calculated using two-way ANOVA, *p < 0.05, cold group vs. normal group; ^#p < 0.05, difference at different time points in the normal group; ^Δp < 0.05, difference at different time points in the cold group; 1 day (1 d), 14 days (14 d), 28 days (28 d), Normal: mice at room temperature (23°C), Cold: mice at 4°C.