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. 2022 Jan 20;12:778019. doi: 10.3389/fendo.2021.778019

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Copyright © 2022 Du, He, Xu, Qu, Cui, Zhang, Zhang, Li and Yu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Brown adipose tissue in the interscapula enlarges in response to cold exposure. (A) Reconstruction images of the micro-CT are shown, brown adipose reconstructs with red and bone with white. (B–G) Body weight, brown adipose volume (BAT volume), lean mass, %lean, fat mass and %fat were calculated. Data are shown as the mean ± SD (n=5 per group). Significance (p-value) was calculated using two-way ANOVA, *p < 0.05; 1 day (1 d), 14 days (14 d), 28 days (28 d), Normal: mice at room temperature (23°C), Cold: mice at 4°C.