Fig. 2. Mena^INV expression in disseminated tumor cells regulates extravasation efficiency.

a Left: Mena^INV expression in extravascular E0771-GFP tumor cells in the lung of an EM model (top) and an SM model (bottom). Green arrow: Mena^INV negative tumor cell. Red arrows: Mena^INV positive tumor cells. Scale bar = 50 μm. Right: Zoomed in view of a disseminated tumor cell (yellow box) in both models. Green = GFP, Red = Mena^INV, White = endomucin, Blue = DAPI. Scale bar = 10 μm. b Quantification of extravascular Mena^INV positive disseminated tumor cells in the lung of each group from Fig. 2a. EM: n = 41 cells in 5 animals. SM: n = 89 cells in 7 animals. Bar = mean. Error bars = ±SEM. Two-tailed Mann-Whitney test (p = 0.0025). **p < 0.01. c Western blot of 231-GFP-Mena11a and 231-GFP-Mena^INV cells. β-Actin was used as a loading control. Full blot is presented in Supplementary Fig. 16. d Outline of experimental design to determine percentage of tumor cells able to extravasate between 0 and 8 h after iv-injection. Intravital imaging of the lung vasculature through the WHRIL was performed before (Timepoint = “Pre”), immediately after (Time point = 0 h), and then finally 8 h after tumor cell injection (Time point = 8 h). e Percentage of tumor cells that extravasated between 0 and 8 h after iv injection. 231-GFP-Mena11a: n = 90 cells in 3 mice. 231-GFP-Mena^INV: n = 88 tumor cells in 3 mice. Bar = mean. Error bars = ±SEM. Two-tailed unpaired t-test (p = 0.0048). **p < 0.01. Source data are provided as a Source Data file.