Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Feb 2;13(2):96. doi: 10.1038/s41419-022-04538-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — A, B Bar plots representing the proportion of γH2AX foci in ALDH^neg (A) and ALDH^br (B) SUM159 cells sorted according to their cell-cycle phases, after 24 h/72 h of treatments with RAD51i or DMSO (CTRL). C–F Quantification of replication-stress markers in ALDH^br and ALDH^neg SUM159 cells treated with RAD51i or DMSO (CTRL). C Representative images of active replication clusters (green staining). Scale bar: 5 μm. D Bee-swarm plots representing the proportion of replication clusters per nucleus in each SUM159 cell subpopulation. E Representative images of combed DNA molecules. CldU (green staining) and IdU (red staining) were detected using specific antibodies and DNA is counterstained with DAPI (blue staining). Scale bar: 10 μm. F Bee-swarm plots representing the distribution of fork speed in each cell subpopulation. G–J Bar plots representing the proportion of RAD51-positive (G, H) or γH2AX-positive cells (I, J) in ALDH^neg (G, I) and ALDH^br (H, J) SUM159 cells sorted according to their cell-cycle phases, after treatment with BMI1i or DMSO (CTRL). K Representative examples of flowchart for the ALDEFLUOR staining following BMI1i or RAD51i treatment in SUM159 cells. DEAB is an ALDH inhibitor used as negative control. L, N Bar plot representing the proportion of ALDH^br cells following BMI1i (L) or RAD51i (N) treatment compared with the untreated condition (CTRL). M, O Bar plot representing tumorsphere-forming efficiency (SFE) for SUM159 cells under BMI1i (M) or RAD51i (O) treatment compared with untreated conditions (CTRL). Statistical test used is Student’s t-test. Data represent mean ± SD.