Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jan 20;8:806215. doi: 10.3389/fcvm.2021.806215

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Kawai, Tohyama, Arai, Tamura, Soma, Fukuda, Shimizu, Nakayama and Kobayashi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Analysis of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) and cardiac organoids (COs). (A) Phase contrast image and immunostaining of purified hiPSC-CMs. (B) Flow cytometry of purified hiPSC-CMs. The proportion of cardiac troponin T-positive cells is >99%. (C) Outline structure of human induced pluripotent stem cell-derived cardiac organoids (hiPSC-COs). They are composed of hiPSC-CMs, human umbilical vein endothelial cells (HUVECs), and normal human dermal fibroblasts (NHDFs) seeded in four different ratios. The hiPSC-COs comprising 50% hiPSC-CMs, 25% HUVECs, and 25% NHDFs show a perfectly circular outline. (D) Beating rate and contraction analysis based on a fold change compared to the minimum area. The beating rate does not vary significantly among the four groups. The extent of contraction is maximum for the hiPSC-COs composed of 100% hiPSC-CMs. (E) Contraction of hiPSC-COs based on a fold change compared to the minimum area. The contraction is stronger in hiPSC-COs that are composed of a higher percentage of hiPSC-CMs. (F) Histological staining with hematoxylin and eosin and immunohistochemical staining for cardiac troponin T, CD31, and CD90.