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. 2022 Jan 20;8:806215. doi: 10.3389/fcvm.2021.806215

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Copyright © 2022 Kawai, Tohyama, Arai, Tamura, Soma, Fukuda, Shimizu, Nakayama and Kobayashi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transplantation procedure and histological analysis of transplanted tubular engineered heart tissue (T-EHT). (A) Scheme of transplantation procedure. The T-EHT is removed from the needle array, cut open, and wrapped around the abdominal aorta and inferior vena cava (IVC). (B) Transplantation procedure. The abdomen of each NOG mouse is opened under general anesthesia. The aorta and IVC are exposed (white arrow) and the T-EHT (black arrow) is wrapped around them. (C) After 1 w of transplantation. T-EHT (black arrow) is engrafted in the NOG mice. (D) and (E) Fluorescent images captured after 1 w of transplantation. The T-EHT and hiPSC-COs were labeled with far-red before transplantation (white arrows). Engrafted tissues with far-red fluorescence were visualized using Nightowl.