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. 2022 Jan 20;8:806215. doi: 10.3389/fcvm.2021.806215

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Copyright © 2022 Kawai, Tohyama, Arai, Tamura, Soma, Fukuda, Shimizu, Nakayama and Kobayashi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histological staining of the tubular engineered heart tissues (T-EHTs) and its high magnifications images after 1 w of transplantation. (A) Hematoxylin and eosin (HE) staining. (B) Myosin light chain 2v (MLC2v) and N-cadherin. (C) CD31 and cardiac troponin T (cTnT). (D) α-actinin and vimentin. (E) MLC2v and cTnT. (F) MLC2v and myosin light chain 2a (MLC2a). The HE staining shows engraftment of the T-EHTs (black arrowheads) (A). Red blood cells were found in the luminal structure with endothelial cells (black arrow). Immune staining reveals MLC2v-positive cells and N-cadherin-positive cells between them (B). The cTnT-positive cells are found in the peripheral area of the tissue, and vimentin-positive cells are found in the middle (C,D). Muscle striations are observed in MLC2a, MLC2v, and α-actinin staining (D–F).