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. 2022 Jan 20;3:795644. doi: 10.3389/fgeed.2021.795644

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Copyright © 2022 Carlsen, Johansen, Yang, Liu, Westberg, Kieu, Jørgensen, Lenman, Andreasson, Nielsen, Blennow and Petersen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Glucan Water Dikinase (GWD) 1—structure and full allelic sequence of gRNA target regions. Overall gene structure with exons (boxes) and the area containing carbohydrate binding module (CBM) depicted above. Left: the nucleotide sequence of exon 1 and introns. Right: exon 24 and 25 including introns. Exons are depicted in capital letters with the amino acid sequence indicated. Small nucleotide polymorphisms (SNPs) from cultivars included in the SPUD database are marked with red, and SNPs found in Saturna are underlined. Grey arrows designate gRNAs (gA, gB, gC, gD, gE, gI, gJ, gK, gL, and gM) with PAM sites marked in bold. Red arrows designate diagnostic IDAA PCR primers. The “CFATC” region, containing cysteine’s hypothesized to be involved in inter or intra-disulfide bond formation and thus in putative redox-state modulation of GWD activity is marked with bold. The active site histidine residue is also marked with bold.