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. 2022 Jan 20;3:795644. doi: 10.3389/fgeed.2021.795644

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Copyright © 2022 Carlsen, Johansen, Yang, Liu, Westberg, Kieu, Jørgensen, Lenman, Andreasson, Nielsen, Blennow and Petersen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Downey Mildew Resistant (DMR) 6—structure and full allelic sequence of gRNA target regions. Overall gene structure with exons (boxes) is depicted above. Left: the nucleotide sequence of exon 1 and 2 with introns. Right: Exon 3 including introns. Exons are depicted in capital letters with the amino acid sequence indicated. Small nucleotide polymorphisms (SNPs) from cultivars included in the SPUD database are marked with red, and SNPs found in Wotan are underlined. Grey arrows designate gRNAs (g43, g44, g45, g46, g47, and g48) with PAM sites marked in bold. Red arrows designate diagnostic IDAA PCR primers. Two of the three catalytic residues histidine H212 and aspartic acid D214 marked with bold. The last catalytic residue H269 located in exon 4 is outside of the figure.